Transition chief John Podesta said Mr Obama would introduce "the strictest, and most far reaching ethics rules of any transition team in history".
He promised the "most open and transparent" ever handover of power.
Mr Podesta gave no indication of when the key posts of treasury secretary and secretary of state would be filled.
Various interest groups have been circulating lists and offering recommendations on cabinet-level appointments to the Obama transition team.
Both Mr Obama, who takes office on 20 January, and his defeated Republican rival John McCain regularly railed against lobbyists during the presidential election campaign.
Favour-peddlers
They accusing them of peddling favours and influence to sway Capitol Hill lawmakers in a culture of corruption.
At a briefing in Washington, Mr Podesta told reporters: "President-elect Barack Obama has pledged to change the way Washington works and curb the influence of lobbyists."
Under the new measures, Mr Obama will not allow lobbyists actively petitioning the federal government to work with the transition, said Mr Podesta.
The guidelines will also prevent anyone who has acted as a lobbyist over the last 12 months from working on any policy area in the transition in which they had been active.
He added that anyone who works on the transition and then becomes a lobbyist will be barred from approaching the administration for 12 months in the area in which they worked.
Mr Podesta also told reporters the future of the Guantanamo Bay detention centre was under review, but that it was a complicated matter, as he put it.
In a previous news briefing, he said Mr Obama would use his executive powers to reverse decisions by outgoing President George W Bush which restricted stem cell research and sought to allow oil drilling in wilderness areas
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